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KMID : 0861020230380050009
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Korea Journal of Herbology
2023 Volume.38 No. 5 p.9 ~ p.19
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The Effect of Ampelopsis japonica (Thunb.) Makino on Osteoclastogenesis and Expression of Osteoclast-Related Gene
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Kim Hong-Sik
Lee Su-Min
Kim Min-Sun
Kim Jae-Hyun
Kang Ye-Jin
Kwon Seoung-Jun
Nam Young-Woo
Yoo Seung-Woo
Choi Hong-Seok
Huh Seon-Jin
Sohn Young-Joo
Jung Hyuk-Sang
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Abstract
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Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased
risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time.
Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose
of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of
Ampelopsis japonica (Thunb.) Makino (AM).
Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast
differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The
impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin
formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast
differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR.
Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore,
AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in
osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK,
MMP-9, CA2, and ATP6v0d2.
Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its
potential for use in the treatment and prevention of osteoporosis.
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KEYWORD
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Ampelopsis japonica (Thunb.) Makino, Osteoporosis, Osteoclast, RANKL, NFATc1, c-Fos
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